TLC Method for Simultaneous Quantification of Chlorzoxazone, Paracetamol, Famotidine and Diclofenac Potassium in their Combined Dosage Form

Abstract

A sensitive, selective and precise high performance thin layer chromatographic method has been developed for the estimation of chlorzoxazone, paracetamol, diclofenac potassium and famotidine in their pharmaceutical dosage form. TLC aluminum plates pre-coated with silica gel 60F 254 used as the stationary phase, while chloroform: methanol: ethyl acetate: hexane: ammonia (10:2.5:1.5:1:0.1, v/v/v/v/v) used as mobile phase. The RF values were observed 0.74±0.01, 0.52±0.01, 0.30±0.01 and 0.14±0.01 for chlorzoxazone, paracetamol, diclofenac potassium and famotidine respectively. The densitometric analysis was carried out in absorbance mode at 282 nm. The method was linear in the range of 250-1500 ng/band for chlorzoxazone, diclofenac potassium and famotidine and 500-3000 ng/band for paracetamol and method was validated as per ICH guideline. The limit of detection and limit of quantitation were found to be 35.98 ng/spot and 109.05 ng/band respectively for chlorzoxazone, 99.74 ng/band and 302.25 ng/band respectively for paracetamol, 58.63 ng/band and 177.69 ng/band respectively for diclofenac and 50.93 ng/band and 154.35 ng/band respectively for famotidine. The proposed method was successfully applied to the quantification of chlorzoxazone, paracetamol, diclofenac potassium and famotidine in their pharmaceutical dosage form.