The Effects of Different Concentrations of Vitamin D3 on Immunological Parameters of Immunosuppressed Rats Induced

Abstract

Vitamin D3 receptor is expressed in several types of immune cells suggesting that Vitamin D3 could have immune regulatory roles. The current study was conducted to investigate the role of Vitamin D3 in reducing the toxicity of the cisplatin on some Immunological parameters in the rat model. The current experiment was conducted on 80 adult white male rats within the age range of 9-12 weeks. The animals were divided into eight groups (10 animals in each group). The control group was dosed with the physiological solution until the end of experiment (C). Rats in the second treatment were injected with cisplatin (2 mg/kg, T1). Rats in the third (T2), fourth (T3), and fifth (T4) groups were injected with cisplatin at a concentration (2 mg/kg) and received Vitamin D3 at levels of 5000 IU, 10,000 IU, and 15,000 IU, respectively. The rats in the sixth (T5), seventh (T6), and eighth (T7) groups were subjected to Vitamin D3 at concentrations of 5000 IU,10,000 IU, and 15,000 IU, respectively. At the end of the experiment, which lasted 21 days, the animals were anesthetized, their weights were recorded, and blood samples were collected. The findings revealed a significant elevation in the levels of interleukin-12, tumor necrosis factor-alpha, C-reactive protein, lymphocyte percentage, monocyte percentage, and eosinophil percentage within group T1 compared to the control and other treatment groups that received Vitamin D3. The average percentage of white blood cells and neutrophils in group T1 was significantly lesser than other groups. It can be concluded that supplementation of different Vitamin D3 levels (5000-10,000 IU) have positive influences on the immunological parameters of immunosuppressed rats.