Validation of the doubly labeled water method using off-axis integrated cavity output spectroscopy and isotope ratio mass spectrometry

Abstract

When the doubly labeled water (DLW) method is used to measure total daily energy expenditure (TDEE), isotope measurements are typically performed using isotope ratio mass spectrometry (IRMS). New technologies, such as off-axis integrated cavity output spectroscopy (OA-ICOS) provide comparable isotopic measurements of standard waters and human(V urine samples, but the accuracy of carbon dioxide production CO2 ) determined with OA-ICOS hasVnot been demonstrated. We compared simultaneous measurement CO2 obtained using whole-room indirect calorimetry (IC) with DLW-based measurements from IRMS and OA-ICOS. Seventeen subjects (10 female; 22 to 63 yr) were studied for 7 consecutive days in the IC. Subjects consumed a dose of 0.25 g H 218 O (98% APE) and 0.14 g 2 H 2 O (99.8% APE) per kilogram of total body water, and urine Vsamples were obtained on days 1 andV 8 to measure average daily CO2 using OA-ICOS and IRMS. CO2 was calculated using bothV the plateau and intercept methods. There were no differences in CO2 measured by OA-ICOS or IRMS compared with IC whenVthe plateau method was used. When the intercept Vmethod was used, CO2 using OA-ICOS did not differ from IC, but CO2 measured using IRMS was significantly lower than IC. Accuracy (~1–5%), precision (~8%), intraclass correlation coefficients (RV = 0.87–90), and root mean squared error (30–40 liters/ day) of CO2 measured by OA-ICOS and IRMS wereV similar. Both OA-ICOS and IRMS produced measurements of CO2 with comparable accuracy and precision compared with IC.