Abstract
Circulating immune complexes (CIC) from 15 paracoccidioidomycosis (PCM) patient sera and from 20 healthy control sera were analysed. After CIC precipitation, solubilization and acid treatment, only a little reactivity to P. brasiliensis antigens was found in the free antibodies from PCM-CIC. This result has suggested that there were antibodies with a high affinity bound to fungus components. Dissociated CIC were fractionated in a column of Sephacryl S300 and the fractions that probably contained antigens were pooled and applied to an affinity column, prepared with mouse anti-gp43 monoclonal antibody. Using ECL-Western blotting assay two polypeptide with apparent mass of 43 and 62 kDa were found.
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CITATION STYLE
Unterkircher, C. S., Yazaki, S. C., Shimizu, M. T., Jorge, A. O. C., & Camargo, Z. P. (1996). Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis. Journal of Medical and Veterinary Mycology, 34(4), 273–277. https://doi.org/10.1080/02681219680000461
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