A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells

Abstract

Background. Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications. Findings. We describe here protocols which adapt baculovirus generation into 96-well format. Conclusion. The established methodology allows simple baculovirus generation, fast virus titering within 18 h and efficient recombinant protein production in a high-throughput format. Furthermore, the produced baculovirus vectors are compatible with gene expression in vertebrate cells in vitro and in vivo. © 2009 Ylä-Herttuala et al.