Threading intercalation to double-stranded DNA and the application to DNA sensing. Electrochemical array technique

Abstract

Electrochemical labeling of double-stranded (ds) DNA is achieved by redox-active DNA intercalators. Threading intercalators derived from naphthalenediimide appended with ferrocene reporter group in the side arms are particularly useful for this purpose. This allows a sensitive electrochemical detection of DNA hybridization on an array, providing a great potential for low-cost, high-throughput, and quick DNA screening technique in post-genome study.