Biogenesis of cocarboxylase in escherichia coli: Regulatory properties of thiamine monophosphate kinase

Abstract

1) Thiamine monophosphate kinase absolutely required a certain monovalent cation, especially K+, for activity. 2) Enzyme activity was inhibited by ATP at high concentrations, and the inhibition was found to be due to a certain degree to the chelating action of Mg++. 3) Enzyme activity was scarcely affected by the intracellular concentration of thiamine pyrophosphate. © 1973, Center for Academic Publications Japan. All rights reserved.