Antioxidant activity of Juglans regia L., Juglandaceae pericarp originated from Sumadija region

Abstract

Objective. The literature provides data on the antioxidant activity of different botanical parts of walnut (Juglans regia L., Juglandaceae) growing in various countries. This research has evaluated the antioxidant activity of pericarp (green husk) of unripe walnut fruit, originated from Sumadija region in Serbia. Methods. The methanol extract of walnut husk was characterized by the total phenol and tannin content, while antioxidant evaluation was done by DPPH radical scavenging assay. Results. It has been found that the antioxidant activity of walnut pericarp is within the range 25.32-79.35 % and is concentration dependent. The IC50 is 1.223 mg/ml. The total phenol content was 119.8 mg GAE/g and tannin 2.645 %. Conclusion. The investigated parameters of the green walnut pericarp from Sumadija region fit the range of values documented for different parts of walnut species growing in other areas in the world.