Pencegahan Infeksi Bakteri Aeromonas hydrophila pada Ikan Lele Dumbo (Clarias gariepinus) dengan Kombinasi Madu dan Patikan Kerbau (Euphorbia hirta)

Abstract

Fish disease is an obstacle that is often encountered in aquaculture activities. The bacteria Aeromonas hydrophila is the bacteria that causes the disease MAS (Motile Aeromonad Septicemia). The use of antibiotics is one way to control fish diseases. However, excessive and long-term use of antibiotics causes bacterial resistance to antibiotics and is not eco-friendly. The use of natural ingredients such as honey and buffalo starch (Euphorbia hirta) as immunostimulants can defend the fish's body against infection with the A. hydrophila bacteria. Honey and buffalo starch (E. hirta) have antibacterial components such as flavonoids, tannins, saponins, terpenoids, polyphenols, hydrogen peroxide and sugar content in honey which can fight bacteria. This research was carried out with the aim of finding out the effect of giving a combination of honey and buffalo patikan (E. hirta) as well as a good comparison of the combination in inhibiting A. hydrophila bacterial infection in African catfish. The research used a Completely Randomized Design consisting of 3 treatment combinations with 3 replications and 2 control treatments (negative and positive) as comparisons. The ratio of combinations used is 2 : 1 (1000 ml honey + 500 ml buffalo patikan boiled water), 1.5 : 1.5 (750 honey + 750 buffalo patikan boiled water) and 1 : 2 (500 ml honey + 1000 ml water buffalo stew). Prevention is carried out by soaking the fish in each treatment ratio for around 1 - 2 minutes for 30 days. The results of the study showed that preventing Aeromonas hydrophila bacterial infection in African catfish using a combination of honey and buffalo starch did not have a real effect in terms of hematological parameters (erythrocytes, leukocytes and hemoglobin), survival and absolute growth parameters. However, clinical symptom parameters show a positive influence as seen from the milder clinical symptoms that arise after bacterial infection.