Conserved cysteine residues provide a protein-protein interaction surface in dual oxidase (DUOX) Proteins

Abstract

Background: Dual oxidases (DUOXs) are membrane-bound ROS-generating enzymes. Results: Conserved DUOX cysteines localized in an N-terminal domain contribute to enzymatic maturation, independent of structural stabilization. Conclusion: Intermolecular disulfides support the interaction between DUOX enzymes and their maturation factors. Significance: This study reflects a complex profile of protein interactions required for activity and localization of the DUOX enzymes. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.