Inhibition of leucyl transfer ribonucleic acid synthetase in Bacillus subtilis by granaticin

Abstract

The bacteriostatic effect of low concentrations of the antibiotic granaticin on B. subtilis is relieved by the addition of leucine to the growth medium. In cells treated with granaticin, aminoacylation of leucine tRNA is specifically decreased, but the content of free leucine is not. It is concluded that granatacin interferes with the charging process of leucine tRNA in B. subtilis leading to leucine auxotrophy.