Analgesic Activity Test 2- (3- (chloromethyl) Benzoiloksi) Benzoate and 2- (4- (chloromethyl) Benzoiloksi) Benzoate on Male Wistar Rats with Plantar Test Method

Abstract

The new compounds of salicylic acid derivatives, 2- (3- (chloromethyl) benzoiloksi)benzoic acid and 2-(4-(chloromethyl)benzoiloksi) benzoate was synthesized to produce a greater analgesic activity and lower stomach irritation from salicylic acid. 2-(4-(chloromethyl) benzoiloksi)benzoic acid and 2-(3-(chloromethyl)benzoiloksi) benzoate was synthesized by Schotten-Baumann acylation reaction. In this study, the analgesic test activity with plantar test method. The compound was given to test animals in doses of 12.5; 25; 50; 100; and 200 mg.kg-1. Analgesic activity was determined by the response time of mice to pain infra-red heat. The percentage is calculated by the mean pain barrier time response to pain. The results showed percentation of pain barrier: 74.28%; 105.58%; 110.58%; 115.29%; and 175.87% after administration of the compound 2-(4-(chloromethyl) benzoiloksi) benzoate at doses of 12.5; 25; 50; 100; and 200 mg.kg-1, respectively. The results of calculations percent pain barrier 2-(3-(chloromethyl)benzoiloksi) benzoate, obtained 85.30%; 92.48%; 124.96%; 180.36%; and 208.01% consecutive for the doses compound of 12.5; 25; 50; 100; and 200 mg.kg-1. Five doses of acetyl salicylic acid ranging from 12.5 mg. kg-1 to 200 mg.kg-1 resulted in percent pain barrier as follows: 26%; 34.34%; 45.68%; 60.38% and 114.12%. This study indicates that 2-(3-(chloromethyl)benzoiloksi)benzoic acid and 2-(4-(chloromethyl)benzoiloksi) benzoate are capable of producing higher analgesic activity than acetyl salicylic acid. Keywords: analgesic, benzoate, plantar test method