Conopeptide Vt3.1 preferentially inhibits BK potassium channels containing β4 subunits via electrostatic interactions

Abstract

Background: BK channel function is differentially modulated by tissue-specific β (β1-4) subunits. Results: Conopeptide Vt3.1 preferentially inhibits neuronal BK channels containing the β4 subunit. Conclusion: Electrostatic interactions between Vt3.1 and the extracellular loop of β4 decrease voltage-dependent activation of the channel. Significance: Vt3.1 is an excellent tool for studying the structure, function, and roles in neurophysiology of BK channels. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.