Effect of Using Two Different Types of Carbon Nanotubes for Blackberry (Rubus adenotrichos) in Vitro Plant Rooting, Growth and Histology

Abstract

See, stats, and : http:// www. researchgate. net/ publication / 276498637 Effect Nanotubes (Rubus) in , Growth ARTICLE DOI : 10 . 4236/ajps.2014.524367CITATION1DOWNLOADS6VIEWS66,INCLUDING:DoraCosta(ITCR)9SEEAvailable:DoraRetrieved:18AbstractNanoparticlesareabletointeractwithbiomolecules,creatingfunctionalnanosystemsfortrans-portationwithininvivocells,andleadingtothestudyoftheirpotentialapplicationsinthefieldofplantbiotechnology.Therefore,theaimofthisresearchwastodeterminethegrowthandrootingeffectoffunctionalized(SWCNTs-COOH)andnon-functionalizednanoparticleswithironresidueinnerparticles(SWCNTs-Fe)inblackberry(Rubusadenotrichos)invitroplants.TwotypesofSWCNTswereused,bothofthemcharacterizedinasolidsamplethroughRamanspectroscopy(λ=532nm)showingdifferencesintheGbandbetweenSWCNT+FeandSWCNT+COOH.Theinvitroplants(approximately15mmlength)wereinoculatedinarootingmedium.Sixtreatmentswereestablished:4,8,12μg/mlforeachtypeofSWCNTsandacontrolwithoutnanotubes.Theassessedvariablesconsistedoftheaveragenumberofdaysforrootemergence,averagenumberofrootsperplant,averagerootlengthperplantandtheaveragestemlength.Thisstudydeterminedthat,ingeneral,theSWCNTs-COOHpromotedthegrowthoftheinvitroplantsunderthisassay,whencomparedtotheSWCNTs-Fetrials.ThelowestSWCNTs-COOHdoseevidencedthebestresultsfortheassessedvariables.Additionally,thehistologicalanalysisalsoevidencedthattheplantstreatedwithSWCNTs-COOHnanotubes(4μg/ml)increasedtheircellularmetabolismwhencom-paredtothecontrolgroup.