FCGR2C genotyping by pyrosequencing reveals linkage disequilibrium with FCGR3A V158F and FCGR2A H131R polymorphisms in a Caucasian population

Abstract

The FCGR3A-V158F and FCGR2A-H131R polymorphisms are associated with clinical responses to therapeutic mAbs and with immune thrombocytopenic purpura (IT P). The FCGR2C-OR F/STO P polymorphism, controlling FcγRII C expression on natural killer cells and therefore FcγRII C-mediated antibody dependent cell-mediated cytotoxicity, is also associated with IT P. Using a new pyrosequencing assay to determine this polymorphism in a control population, we observed the expected allele frequencies (OR F:12.6%) and percentages of individuals with a single copy (10.0%) or 3 copies (12.1%) of FCGR2C, or with at least one FCGR2C-OR F allele (20.1%). No association of FCGR2C copy number variations with the FCGR3A-V158F or FCGR2A-H131R genotype was detected. More importantly, our results demonstrate a strong and a weaker linkage disequilibrium associating the FCGR2C-OR F allele with the FCGR3A-158V and the FCGR2A-131H allele, respectively. © 2012 Landes Bioscience.