Research on the Establishment of Strawberry Stem Tip Tissue Cultivation and In-vitro Rapid Micropropagation System

Abstract

The creeping shoot tips of strawberry varieties “Shengdanhong” and “Hongyan” were cultivated in vitro to develop protocols for expeditious and reliable micropropagation. The morphogenetic response of varying concentrations and combinations of different hormones on shoot tip germination, proliferation and rooting were studied. The results showed that the most suitable shoot tip induction medium for “Shengdanhong” was MS media containing 0.5 mg·L-1 6-BA and 0.2 mg·L-1 IBA, while for “Hongyan” it was MS media containing 1.0 mg·L-1 6-BA and 0.1 mg·L-1 IBA, with corresponding germination rates of 84.21% and 88.10%. The optimal subculture adventitious bud proliferation outcome had been cultured in MS media supplemented with 0.2 mg·L-1 6-BA and 0.1 mg·L-1 NAA, with proliferation coefficients were 6.11 and 7.13, respectively. The highest corresponding rooting rates were 72.41% and 91.67% for “Shengdanhong” and “Hongyan”, as cultured in 1/2MS medium containing 0.5 mg·L-1 IBA and 2 mg·L-1 AC, and 1/2MS containing 0.5 mg·L-1 IBA, respectively. Virus detection of regenerated seedlings by RT-PCR showed that the SMoV and SCV detoxification rates were 92.5% and 100%, respectively. These examined findings contribute valuable insights into the mass production of virus-free strawberry plantlets using in vitro culture techniques.