Occurrence of a subgroup 16SrIII-J phytoplasma in non-symptomatic Brachiaria decumbens cultivated in a grazing area

Abstract

Brachiaria spp. are the most important and widely used warm season grasses for pasture, but are also important weeds for most economic crops in the tropics. During phytoplasma surveys in pasture fields, asymptomatic plants of Brachiaria decumbens were collected for inspection. Nested PCR detected phytoplasmas in more than 90% of sampled plants. Based on computer-simulated RFLP and a similarity coefficient calculation, a subgroup 16SrIII-J phytoplasma was molecularly characterized. Phylogenetic analysis was consistent with virtual RFLP identification. Representatives of this subgroup have been identified in diverse Brazilian regions encompassing various commercial crops. Our study revealed B. decumbens as a new host and reservoir of 16SrIII-J phytoplasma.