Influence of biotic elicitor Aspergillus Niger on salicylic acid products in callus cultures of Calendula officinalis L. plant

Abstract

The study was carried out in the college of education for pure sciences / department of biology / plant tissue and cell culture laboratory to study the effect of biotic elictors Aspergillus Niger in the production of salicylic acid in callus cultures of Calendula officinalis L. plant. the results showed the best induction of the explants, which included the leaves and cotyledon obtained from culturing on the solidified MS medium supplemented with 2.0 mg.L1- of 2,4- Dichlorophenoxy acetic acid (2,4-D) interacted with 0.5 mg.L-1 Kinetin, which reached the highest fresh weight of callus was 2.2350 and 2.7125 g, respectively, after four weeks culture. The callus induction from the leaves its compact, in contrast to the callus induced by the cotyledon, which is characterized its friable.