Mycobacterium avium subsp. paratuberculosis (MAP) en un hato lechero de búfalos en Colombia

Abstract

Objective. To determine the presence of Mycobacterium avium subsp. paratuberculosis (MAP) using serological, microbiological, and molecular methods, to genotype MAP isolates, and to explore factorsassociated to MAP-seropositive status in a buffalo herd of the province of Antioquia (Colombia).Materials and methods. The study was carried out to test fecal and serum samples from 21 asymptomatic adult buffaloes using culture and ELISA tests. Suspicious culture isolates were confirmedby MAP-IS900-PCR (IS900-PCR). The positive DNA was retested using MAP-IS900-quantative PCR(IS900-qPCR), and then, sub-typed using techniques based on the detection of mycobacterialinterspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) and multilocus shortsequence repeat (MLSSR). Results. At sampling, the average age of animals was 4.7 years, 90.5%(19/21) were females and 84.2% (16/19) were pregnant. The average body condition score was4.2 (in a 1-to-5 scale). The average milk yield was 3.5 L/day/animal. No MAP-seropositive animalswere detected. One dairy buffalo cow out of the 21 buffaloes was positive by fecal culture at week 6of incubation and then confirmed by IS900-PCR. Sub-typing analysis revealed that the MAP isolatecorresponded to the INMV 3 genotype by MIRU-VNTR. Conclusions. To the best of the author’sknowledge, this is the first report of MAP diagnosis and isolation in Colombian buffaloes and the firsttime an INMV 3 MAP-profile is found in this species the country