Identification of Secondary Metabolites from Callus Orthosiphon aristatus (Blume) Miq by Thin Layer Chromatography

Abstract

The flowers of Orthosiphon aristatus are purple, white-purple, and white. The main chemical compounds of O. aristatus are rosmarinic acid, eupatorin and sinensetin. The O. aristatus plant's potential as traditional medicine is established, so it is necessary to produce its active compounds abundantly and to propagate purple and white-purple varieties of O. aristatus. A strategy that can be adopted to achieve plant tissue culture (callus induction). This research aim is to identify secondary metabolite in callus using thin layer chromatography (TLC). The profiling of ethanol extracts of callus of two varieties O. aristatus were carried out using solvent system toluene-ethyl acetate- formic acid-water (3:3:1:0.2). The results of monitoring TLC suggest that callus obtained from Schenk and Hildebrandt (SH) media can proceed to the suspension culture stage because it showed brighter fluorescence spot than other callus and plant extracts, especially for rosmarinic acid. This research provides new information about secondary metabolites in callus derived from two varieties of O. aristatus on various growth media.