The effects of mycorrhizal colonization on phytophagous insects and their natural enemies in soybean fields

Abstract

The use of belowground microorganisms in agriculture, with the aim to stimulate plant growth and improve crop yields, has recently gained interest. However, few studies have examined the effects of microorganism inoculation on higher trophic levels in natural conditions. We examined how the diversity of phytophagous insects and their natural enemies responded to the field-inoculation of soybean with a model arbuscular mycorrhizal fungus (AMF), Rhizophagus irregularis, combined with a nitrogen-fixing bacterium, Bradyrhizobium japonicum, and a plant growth-promoting bacterium, Bacillus pumilus. We also investigate if the absence or presence of potassium fertilizer can affect this interaction. We found an increase in the abundance of piercing-sucking insects with the triple inoculant irrespective of potassium treatment, whereas there were no differences among treatments for other insect groups. A decrease in the abundance of the soybean aphid, Aphis glycines, with the double inoculant Rhizophagus + Bradyrhizobium was observed in potassium enriched plots and in the abundance of Empoasca spp. with potassium treatment independent of inoculation type. Although it was not possible to discriminate the mycorrhization realized by inoculum from that of the indigenous AMF in the field, we confirmed global negative effects of overall mycorrhizal colonization on the abundance of phytophagous piercing-sucking insects, phytophagous chewing insects, and the alpha diversity of phytophagous insects. In perspective, the use of AMF/Rhizobacteria inoculants in the field should focus on the identity and performance of strains to better understand their impact on insects.