Hypocholesterolaemic effect of dietary inclusion of two putative probiotic bile salt hydrolase-producing Lactobacillus plantarum strains in Sprague-Dawley rats

Abstract

The purpose of the present study was to evaluate the anti- hypercholesterolaemic effects of two putative probiotic bile salt hydrolase (Bsh)-producing Lactobacillus plantarum strains, i.e. Lp91 and Lp21, in rats. L. plantarum Lp91 exhibited excellent tolerance to low pH and high bile salt concentrations as well as showed potential Bsh activity, cholesterol assimilation and cholesterol co-precipitation ability along with L. plantarum Lp21 and NCDO82 strains. Furthermore, the potential effect of L. plantarum Lp91 on plasma cholesterol level was evaluated in Sprague-Dawley rats. Five treatment groups of rats (n 6) were fed experimental diets: normal diet, hypercholesterolaemic diet (HD), HD plus L. plantarum Lp91 (HD91) at1010 8 colony-forming units (cfu)/g, HD plus microencapsulated L. plantarum Lp91 (HDCap91) at10108cfu/g and HD plus L. plantarum Lp21 (HD21) at10108cfu/g for 3 weeks. Feed intake and feed efficiency differed significantly among the five groups. After 21d of dietary treatment, comparative analysis revealed 2326, 1571 and 1501% reduction in total cholesterol, 2109, 1877 and 1817% reduction in TAG, 3813, 2322 and 2142% reduction in LDL-cholesterol, and the corresponding HDL-cholesterol values increased at the rate of 1894, 1030 and 778% in treated groups HD91, HDCap91 and HD21, respectively. Faecal excretion of cholic acid and faecal lactobacilli counts were significantly higher in the probiotic treatment groups than in the control groups. In conclusion, these results suggest that the indigenous L. plantarum Lp91 strain has the potential to be explored as a probiotic in the management of hypercholesterolaemia. © 2010 The Authors.