Structural Analysis of a Glycoside Hydrolase Family 11 Xylanase from Neocallimastix patriciarum

Abstract

Background: Thermophilic xylanases are valuable in many industrial applications. Results: The structures of a xylanase XynCDBFV and its complex with xylooligosaccharides were determined, and its N-terminal region (NTR) contributes to thermostability. Conclusion: NTR may stabilize the overall protein folding of XynCDBFV. Significance: The structural and functional investigation of unprecedented NTR of XynCDBFV provides a new insight into the molecular basis of thermophilic xylanases. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.