Atividade antibacteriana "in vitro" de diferentes acessos de urucum (Bixa orellana L.) e sua relação com o teor de bixina presente nas sementes

Abstract

In the present study were determined the intensity of bacterial inhibition activity (IINIB/bacteriostasy) and the intensity of bacterial inactivation activity (IINAB/bactericidie) from solutions containing hidroetanolic and hydric (decoction and infusion) extracts of tree accesses from Bixa orellana L. (annatto) from Arroio do Meio (RS- Brazil), Eldorado do Sul (RS - Brazil) and Maringá (PR - Brazil), on standardized inocula of Salmonella enteritidis (ATCC 11076), Escherichia coli (ATCC 11229), Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 19433) and Listeria monocytogenes (ATCC 19114). In parallel, the content of bixin present in the seeds was also determined. The forms of hydric extraction presented low inhibition and/or inactivation activity of the bacterial inocula. Hidroetanolic extract form on the other hand showed selective antibacterial activity and signifi cantly pronounced inhibition/inactivation against the fi ve bacteria tested. Independent of the extraction form, E. faecalis and L. monocytogenes were the more sensitive agents to the antibacterial activity. E. coli had the lowest sensitivity to all forms of extraction. The bixin contents were signifi cantly different among the three accesses and, consequently, antibacterial activity was directly proportional to these contents.