MiR-542-3p overexpression is associated with enhanced osteosarcoma cell proliferation and migration ability by targeting Van Goghlike 2

Abstract

Osteosarcoma is the most common histological form of primary bone cancer, which arises from osteoid tissue. It occurs predominantly in infants and adolescents, with an incidence of 45 cases/100,000,000. The 5-year survival rate of patients with osteosarcoma has significantly improved over time; however, there remains a significant proportion of patients that respond poorly to chemotherapy. An improved understanding of the pathology of osteosarcoma is required to provide more effective treatment strategies, identify biomarkers and develop novel chemotherapeutic agents. Disturbance in microRNA (miRNA) expression has been identified in osteosarcoma tissues and cell lines; however, the roles of miRNA during osteosarcoma pathogenesis remain to be elucidated. In the present study, the expression levels of eight selected miRNAs were investigated in osteosarcoma tissues and the results revealed that the expression levels of miR5423p and miR5425p were significantly upregulated and the expression of miR1993p was significantly downregulated. Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Goghlike 2, which is a noncanonical Wnt pathway suppressor, was a target gene of miR5423p. Subsequently, the biological function of miR5423p in U2OS cells was examined, which revealed that overexpression of miR5423p can enhance the cell proliferation and migration ability of U2OS cells. This indicated that miR5423p may act as an oncogene in osteosarcoma pathogenesis. The findings of the present study may provide assistance in understanding the development of osteosarcoma and aid in the development of strategies for the diagnosis and treatment of osteosarcoma.