Transcriptional profiling of spleen lymphocyte in fowl typhoid of broilers

Abstract

This study was carried out to investigate the differentially expressed genome between S. gallinarum infected and uninfected control in the spleen lymphocytes of Ross broiler chicks using microarray analysis. GeneChip Chicken Genome Array containing 32,773 transcripts corresponding to over 28,000 chicken genes for simultaneous expression was used. The signal intensity of each gene was normalized and expressed in fold change. A large numbers of genes were found with differential expression majority of which are still unknown in chicken genome. Thirty one known genes were found to have differential expression of which, 25 were up-regulated and 5 were down regulated. Majority of the up-regulated genes belong to immune response system viz., IL8, IL 1B, IL10, IL18, IL17A, IL15, transferrin, IFNg, TLR2, TNFRSFIb, TNFRSF15 and the down regulated genes were B-FIV, B-LA, SDF1, B-LBL belonging to MHC-I and II and CD1d. To validate the expression of these genes RT-PCR was done using primers of 12 selected genes' with total mRNA isolated from spleen lymphocytes which has confirmed the similar pattern of expression of all the genes as in microarray. The findings in this study have lead to the identification of novel genes which may be useful in further studies to understand the pathophysiology of fowl typhoid towards development of diagnostics and therapeutics. © 2009 Academic Journals Inc.