Jaeumganghwa-tang, a traditional herbal formula inhibits the development of benign prostatic hyperplasia in rats

Abstract

Benign prostate hyperplasia (BPH) is the most common proliferative disorder affecting older men and results in prostate enlargement and lowered urinary tract symptoms. Jaeumganghwa-tang (JGT), an oriental traditional herbal formula, has been used in China (Zi-yin-jiang-huo-tang in Chinese), Japan (Jiin-koka-to in Japanese), and Korea for many years. Effects of JGT on prostate dihydrotestosterone (DHT) level and prostatic hyperplasia were investigated using a rat model, in which BPH was induced using testosterone propionate (TP). Rats were divided into five groups. One group was used as a normal, and four groups received s.c. injections of TP for 4 wk to induce BPH. JGT (200 or 400 mg/kg) was administered daily for 4 wk by oral gavage concurrently with TP injections, and rats were sacrificed at scheduled times. Prostates were weighed, and histopathol. examn. was conducted. DHT levels in serum and the prostate were measured, and the expression of proliferating cell nuclear antigen (PCNA) protein was investigated using Western blotting. BPH animals showed increases in abs. and relative wts. of the prostate, levels of DHT in serum and the prostate, and expression of PCNA in the prostate, whereas JGT-treated animals showed significant redns. in these indexes compared with the BPH animals. Administration of JGT attenuated TP-induced epithelial hyperplasia. These findings indicate that JGT inhibits the development of BPH, an effect closely assocd. with a redn. in DHT level. [on SciFinder(R)]