Green-synthesis of Platinum Nanoparticles using Olive Leaves Extracts and its Effect on Aspartate Aminotransferase Activity

Abstract

This study aims to synthesize Platinum nanoparticles (Pt NPs) using Olive leaves extract's environmentally friendly approach. UV-Visible spectroscopy has been used to follow the turning of Pt +4 ions to Pt0 NPs. X-ray diffraction (XRD) has been used to investigate the as-synthesized Pt NPs and its cubic face-centered structure crystallinity. The average size of nanoparticles was 9.2 nm, calculated by transmission electron microscopy (TEM). The activity of as-synthesized Pt NPs has been examined by inhibition of Serum Aspartate Aminotransferase (AST) level in patients with chronic liver disease and control group, The AST (mean= 12.8051.642) demonstrate a very significant rise (p<0.01) in the results. In chronic liver patients with Pt NPs, serum AST activity was significantly lower (p<0.01) than in patients without Pt NPs (mean=13.4582.360). The present study concluded that Platinum nanoparticles play a great role in inhibition of AST.