Fast determination of naringin and hesperidin in Natural and Commercial Citrus Juices by HPLC Method

Abstract

A reversed-phase high performance chromatographic method for simultaneous determination of naringin and hesperidin in natural and commercial citrus juices was developed. The separation was achieved on Zorbax Eclipse XDB C18 column (4.6 mm × 250 mm, 5 μm) using a acetonitrile:water:formic acid (21:78.8:0.2) solution at pH 2.5 as a mobile phase under isocratic conditions. Analysis was run at a flow rate of 1 mL min-1 with a 15 min total run time at room temperature. The detection wavelength was set at 280 nm. Linearity of calibration, accuracy, precision were examined as parts of the method validation. The optimized method was applied to analysis of natural and commercial citrus juices. Commercial fruit juices contained lower amount of naringin and hesperidin compared with natural fruit juices.