Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase- and AmpC β-lactamase-producing enterobacter spp. Isolated from different clinical samples in Tehran, Iran

Abstract

Introduction: Here, we determined the genes encoding antibiotic resistance enzymes and virulence factors and evaluated the genetic relationship between Enterobacter spp. isolated from different clinical samples. Methods: A total of 57 clinical isolates of Enterobacter spp. were tested for the production of extended-spectrum β-lactamases (ESBLs), carbapenemase, and AmpC using phenotypic and genotypic methods. Results: The most common ESBLs and AmpC β-lactamases were blaTEM (63.3%) and blaEBC (57.7%), respectively. The most prevalent virulence gene was rpos (87.7%). The random amplified polymorphic DNA (RAPD) patterns of strains were genetically unrelated. Conclusions: RAPD polymerase chain reaction analysis revealed high genetic diversity among isolates.