Stabilization of Actin Filaments by ATP and Inorganic Phosphate

Abstract

Both inorganic orthophosphate and ATP stabilize actin filaments. This is reflected by a reduced nucleotide exchange and by a protection against filament breakdown by SDS or KI. When the filament-stabilizing effect of ATP was maximal, only about 15% of the actin subunits of the filament had bound one molecule of the nucleotide offered in the stabilizing solution. © 1989, Verlag der Zeitschrift. All rights reserved.