The effects of colocasia esculenta leaf extract in inhibition of erythrocyte aldose reductase activity and increase of haemoglobin in experimental rats

Abstract

Diabetes Mellitus (DM) has reached a number of 382 million in 2013 and expected to rise to 592 million by 2035. Chronic diabetes can lead to impaired formation of erythropoietin in haemoglobin production and may cause anemia. Inhibition of aldose reductase is a key point of diabetes treatment and prevention of complications in diabetes. Colocasia esculenta (CE) leaf is one of Indonesian vegetables which has inhibition effect on aldose reductase activity. This research was a true experimental study with post-test only group design. 21 male Sprague dawley rats were divided into: K (control group), P1 (extract CE 200 mg/KgBW) and P2 (extract CE 400 mg/KgBW). Rats were induced to become obese with High Fat Sucrose Diet (HFSD) for 4 wk then extract CE were given for 3 wk. The data were analyzed with independent t-test. CE have a significant effect to increase haemoglobin but have no significant inhibition effect to erythrocyte aldose reductase activity. The results of this research found that the mean haemoglobin of control group was 13.14±1.55, treatment group 1 (P1) was 15.22±0.59, and treatment group 2 (P2) was 15.77±0.71. There was significant increase in haemoglobin (p<0.05). The mean of aldose reductase activity of treatment group was lower than control group. However, there was no significant difference found (p>0.05) between the groups. 200 mg/kgBW and 400 mg/kgBW dose of CE could increase haemoglobin and decrease the mean of aldose reductase activity.