Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique

Abstract

This study carried out to direct molecular investigation of Entamoeba bovis from feces samples of calves which suffering from diarrhea that collected from different fields in  Al-Diwanyia city by using polymerase chain reaction technique (PCR). This technique was dependent on used specific primers that amplification of  small subunit ribosomal RNA gene in Entamoeba bovis. This primers were designed in this study by using NCBI-Genk data base (FN666248.1) and primer 3 plus for primers design. The PCR results were appeared that cattle infected with Entamoeba bovis in percentage of about (36% ) 18 positive samples out of 50 diarrheic  samples. We concluded that Entamoeba bovis is important causes of enteric infection in calf whereas, the polymerase chain reaction technique is very specific and rapid assay.