Influence of alcohol: oil molar ratio on the production of ethyl esters by enzymatic transesterification of canola oil

Abstract

See, stats, and : https : / / www. researchgate . net / publication / 284198124 Influence : oil production transesterification Article DOI : 10 . 5897 / AJB2013 . 12313 READS 9 7 , including : Carlos Universidade 10 SEE Pedro Universidade 83 SEE Cleide Universidade 96 SEE Gisella Universidade 140 , 500 SEE All - text , letting . Available : Pedro Retrieved : 12 The influence of alcohol : oil molar ratio on the canola oil transesterification reaction in solvent - free medium using free lipase from Thermomyces lanuginosus and Burkholderia cepacia was studied . The experiments conducted in batch reactor for 72 h at 37°C in cosolvent - free reaction system with ethanol addition in three steps showed great potential for ester production . The stepwise addition of ethanol allowed increasing yield throughout the total period of the reaction , even if the course has limited reaction at times , minimizing possible deleterious effects of the alcohol on the enzyme structure . The highest yields were achieved with lipase from T . lanuginosus , despite presenting lower activity values than those of Burkholderia cepacia lipase , which proved to be less selective for ester production . In the reaction medium containing lipase from T . lanuginosus , 100 % yield was obtained using a molar ratio of 12 : 1 . For B . cepacia lipase , the highest yield was 90 . 73% at a molar ratio of 6 : 1 . In all cases studied , at least 92% of the triacylglycerols from canola oil were consumed .