Preliminary Screening and Phytochemical Profile of Mangifera indica Leave's Extracts, Cultivated in Iraq

Abstract

The main objective of this study was to evaluate the Mango leaves that recently cultivated in Iraq, depends on preliminary investigation and phytochemical profiles of Mangifera indica leaves (F.Anacardiaceae) extracted with different polar solvents. Then we chose ethyl acetate, the most appropriate solvent to obtain maximum amount of phenolic compounds from mango leaves so that it can be used as nutritional supplement. The preliminary phytochemical screening was performed by the standard methods as described by Harborne. These tests indicate the presence of alkaloids, Tannins, flavonoids, steroids and absence of saponin glycosides. In addition Four different leaf extracts (methanol, ethanol, nbutanol and ethyl acetate) were analyzyed by Preperative high performance liquid chromatography (PHPLC) to identify bioactive compounds. The results confirmed that Mangifera indica leaves accumulate large number of secondary metabolites in different percentages, but the higher percentage related to flavonoid glycosides. So that we analyzed the ethyl acetate extract of fresh mango leaves by GC-MS and the result show high percentage of quercetin. These findings will be helpful to the phytochemists and pharmacologists for identification of new active principles in future.