Constitutive activation of nuclear factor jb contributes to cystic fibrosis transmembrane conductance regulator expression and promotes human cervical cancer progression and poor prognosis

Abstract

Objective: Cystic fibrosis transmembrane conductance regulator (CFTR) and nuclear factor JB (NF-JB) have been known to play important roles in the development and progression of many types of cancer including cervical cancer. The study aimed to verify the relevance and significance of CFTR and NF-JB expressions in cervical cancer tissues and cell lines. Methods: The expressions of CFTR and NF-JB p65 were analyzed respectively by immunohistochemistry in total of 135 cervical tissue samples. The correlation to clinicopathologic characteristics and prognostic value was evaluated. The coexpression of CFTR and NF-JB was detected in cervical cancer cell lines. Nuclear factor JB signaling was inhibited by siRNA for NF-JB p65 and activated by stimulation of cells with interleukin A or tumor necrosis factor