Development of enterobacterium-specific oligonucleotide probes based on the surface-exposed regions of outer membrane proteins

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Abstract

Outer membrane proteins of members of the family Enterobacteriaceae consist of conserved membrane-spanning segments and hypervariable, surface-exposed regions. We demonstrate that the hypervariable DNA segments corresponding to the surface-exposed regions of these proteins can be used to develop specific DNA probes for the identification of members of the family Enterobacteriaceae.

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Spierings, G., Hofstra, H., Huis in ’t Veld, J., Hoekstra, W., & Tommassen, J. (1989). Development of enterobacterium-specific oligonucleotide probes based on the surface-exposed regions of outer membrane proteins. Applied and Environmental Microbiology, 55(12), 3250–3252. https://doi.org/10.1128/aem.55.12.3250-3252.1989

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