Determination of Ciprofloxacin in Pharmaceutical Preparations Using (ZIC-HILIC) with UV Detection

Abstract

Hydrophilic interaction chromatography (HILIC) method was developed to quantify ciprofloxacin in pharmaceutical samples using two ZIC1-HILIC and ZIC4-HILIC exchangers. These exchangers were produced by grafting polymerization a sequence of sulfobetaine precursors onto the surface of porous PS/DVB particles. The separation was carried out with the mobile phase acetonitrile and acetate buffer (85:15), flow rate of 0.5 mL/min and detected at 273 nm was used. The various spacer lengths were used to investigate the retention behavior of ciprofloxacin. It should be noted that an increase in the length of the chain leads to an increase in the ciprofloxacin retention time, as indicated in ZIC4-HILIC exchanger. The retention behavior of ciprofloxacin with two exchangers was investigated, and the findings identified the hydrophilic interactions. The calibration curves for ciprofloxacin with two exchangers over a concentration range from 0.05 to 9 μg mL-1, LOD (0.008 and 0.005 μg mL-1), LOQ (0.024, 0.015 μg mL-1), respectively. The proposed method has been shown to be appropriate for pharmaceutical samples. Additionally, the proposed method's results are compared to those obtained using the standard method, and their precision and accuracy are comparable.