Analysis of large DMA from soybean (Glycine max L. Merr.) by pulsed‐field gel electrophoresis

Abstract

The technique of pulsed‐field gel electrophoresis (PFE) has been used to study chromosomal regions and entire genomes of several organisms. Techniques are presented for the isolation of high molecular weight DMA from embedded soybean protoplasts and the conditions for separating large DMA fragments using PFE. Digestion was detected by Southern hybridization using single copy nodulin clones. These data are being used to generate a physical map of the nodulin region(s) of the soybean genome. Copyright © 1992, Wiley Blackwell. All rights reserved