Inhibitory effect of sargassum fulvellum ethanolic Extract on LPsinduced inflammatory reaction in RAW 264.7 mouse macrophages

Abstract

Recently, algae has been considered as a potential antiinflammatory source due to its distinctive habitat environment exposing to light and high oxygen concentration. In present study, anti-inflammatory effect of brown alga, Sargassum fulvellum ethanol extract (SFEE), was examined. SFEE inhibited not only the production of nitric oxide and pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) but also the expression of inducible nitric oxide synthase and cyclooxygenase 2 in LPS-induced RAW 264.7 cells without affecting cell viability. SFEE also suppressed the expression of nuclear factor kappa B (NF-κB), suggesting that SFEE could affect the expression of inflammation related cytokines and proteins through the regulation of NF-κB. Furthermore, formation of edema of the ear was 40% lower in mice treated with the highest dose (250 mg/kg) of SFEE than in the control mice. Thus, our study showed that SFEE may be a potential therapeutic anti-inflammatory drug. © The Korean Society for Applied Biological Chemistry 2013.