Isolation and identification of endophytic fungi in walnut

Abstract

In this paper, endophytic fungi in four tissues (roots, branches, leaves, fruits) of walnuts were isolated by tissue isolation technic. Based on morphology, the isolated endophytic fungi were preliminarily identified. The results showed that 64 strains of endophytic fungi were isolated from walnut tissues. The number of endophytic fungi isolated from walnut branch tissues is the largest, followed by leaf, fruit, and root tissues. Observing the morphological and mycelial changes during fungal growth and the characteristics of spore morphology under the microscope, 64 strains of endophytic fungi belonged to 17 genera, of which Alternaria sp. was the dominant microflora. For the first time, Talaromyces sp., Curvularia sp. and Eurotium sp. were isolated from walnut plants.