Response of Blood Metabolite Levels of Saanen-Etawah Crossbred Does to Ovarian Cycle

Abstract

The physiological state during the estrus cycles has the potential to affect the blood biochemical composition of livestock and serve as a reference for livestock reproductive nutrition and maintenance management. This study aims to determine the blood metabolite levels of Saanen-Etawah Crossbred does during the follicular and luteal phases. Fourteen non-pregnant-lactating Saanen-Etawah Crossbred does were used in this study (age 2–4 years, BCS 3, and multiparous). The same management was given throughout this research and the feed intake was observed daily. The blood was taken during the follicular and luteal phase which was validated from data of vaginal smear. The obtained serum from whole blood was immediately for total protein, cholesterol, glucose, and blood urea nitrogen (BUN) levels assay. The spectrophotometry method used for biochemical data using specifics enzymatic were used. Feed samples were analysed using proximate method to determine dry matter (DM), crude protein (CP), and total digestible nutrient (TDN) intake. The data results were analysed using Independent Sample T-Test and the correlation between blood metabolite profiles and nutrients was analysed using bivariate correlations by Pearson. The data showed that the cholesterol level was higher (p<0.05) on follicular phase (134.04±25.11 mg/dL) than on luteal phase (96.74±22.67 mg/dL), while no differences were found in other parameters despite slight discrepancies between the two phases. During the follicular phase, there was a strong correlation (p<0.01) between respective parameters such as BUN with glucose and DM with TDN intake. However, the correlation data did not show any differences in the luteal phase.