Chlorogenic acid prevents isoproterenol-induced DNA damage in vascular smooth muscle cells

Abstract

Numerous clinical therapeutic agents have been identified as DNA damaging. The present study revealed that isoproterenol (Iso) resulted in DNA damage in vascular smooth muscle cells (VSMCs) and increased the levels of intracellular oxygen free radicals. Administration of chlorogenic acid (CGA) inhibited this effect. Pretreatment with CGA abrogated the increase in protein expression levels of H2A histone family member X, phosphorylated ataxia telangiectasia mutated, phosphorylated Rad3related protein, breast cancer 1 and Cterminal Src homologous kinase induced by Iso. In addition, the increase in levels of intracellular reactive oxygen species (ROS) induced by Iso was inhibited by CGA pretreatment in a dosedependent manner. The results of the present study suggest that CGA may inhibit Isoinduced VSMC damage via the suppression of ROS generation. Therefore, CGA may be a novel agent for the treatment of vascular diseases.