Advances in magnetic particle imaging: evaluating magnetic microspheres and optimized acquisition parameters for high sensitivity cell tracking

Abstract

IntroductionThe sensitivity and resolution of magnetic particle imaging (MPI) depend on the choice of tracer and specific imaging parameters. For cell tracking applications with MPI, both the superparamagnetic iron oxide (SPIO) tracer and the cell labeling efficiency have a significant impact on MPI sensitivity and vary for different tracers.MethodsThis study compared three commercially available SPIO tracers (VivoTrax, Synomag-D and ProMag) and SPIO-labeled cells using magnetic particle relaxometry (MPR) and imaging. Further, the effect of imaging parameters (high and low gradient field strength and drive field amplitude) on MPI signal strength, resolution, and cell detection limits, was evaluated.ResultsThe peak MPI signal measured by MPR was much higher for Synomag-D compared to VivoTrax and ProMag. However, the signal for intracellular Synomag-D was significantly reduced. In contrast, the signal for ProMag, a micron-sized iron oxide (MPIO) particle, was not significantly different for free and intracellular particles. The cellular iron loading was higher for ProMag compared to Synomag-D. The total MPI signal measured from images of free and intracellular SPIOs was highest for ProMag. Varying imaging parameters confirmed that a lower gradient field strength and higher drive field amplitude improved tracer and cellular sensitivity.DiscussionThese results, in addition to prior work from our lab, suggest that MPIOs are a good option for cell tracking with MPI. In conclusion, the evaluation of tracers by MPR is not sufficient to predict the performance of all SPIO tracers; in particular, not for larger, polymer-encapsulated iron particles such as ProMag, or for SPIO tracers internalized in cells. Improvements in MPI sensitivity through lower gradient field strength and higher drive field amplitudes are associated with a trade-off in image resolution.