Extraction and profiling of proteins in yellow powder from sweet potato starch wastewater using response surface methodology and proteomic approach

Abstract

Abstract: Sweet potato starch industry produce generous high soluble solid wastewater containing various biochemicals such as proteins. The wastewater could be spray dried into a product called yellow powder (YP). Proteins in the YP were recovered and profiled in this study. The extraction conditions were optimized on dependent variables of YP material–water ratio, pH, and temperature using response surface methodology (RSM). Maximum protein yield (61.2%) using RSM were observed at a material–water ratio of 50 (mg/L), pH 9.5, and extraction temperature of 30℃. Subsequently, a total of 25 proteins were identified by proteomic analysis, which mainly were sporamins, β-amylase, starch phosphorylase, polyphenol oxidase, and superoxide dismutase. The extraction and profiling of proteins from YP would contribute to a comprehensive utilization and added value of the wastewater produced by sweet potato starch processing industry. Practical Application: This study reported the recovery (61.2%) of proteins and protein profile of yellow powder (byproducts) from sweet potato starch wastewater. These information could contribute to the valorization a yellow powder into high-value ingredients.