On the Subzero Temperature Preservation of Fertilized Eggs of Rainbow Trout

Abstract

A possibility of preservation of fertilized eggs of rainbow trout Salmo gairdneri was examined at −7°C 20°C for 3 hours. The results obtained are summarized as follows; 1. Eggs after eyed stage had higher tolerance for subzero temperature exposure than those of before eyed stage. 2. Suitable concentration of dimethyl sulfoxide (DMSO) as organic cryoprotectant was 1.4–2.1m. 3. In −7°C exposure, about 95% of hatching rate was obtained by using 10% calf serum and mineral salts solution besides DMSO as cryoprotectants, while 14% of hatching rate was obtained in −12°C exposure. © 1982, The Japanese Society of Fisheries Science. All rights reserved.