Dual-phase stimulated Raman scattering microscopy for rapid, label-free histology

Abstract

Stimulated Raman scattering (SRS) microscopy is an emerging label-free imaging technique. It is a nonlinear version of Raman scattering, with the advantages of high chemical selectivity and rapid imaging. We have applied SRS microcopy for brain tumor detection on both in vivo mouse models and ex vivo human surgical specimens, based on the spectral differences between normal and tumor tissues. We further developed dual-phase parallel imaging technique for real-time two color SRS imaging, which provides a key advance for intraoperative virtual histology.