Comparative Analysis of Antioxidative Activity of Flavonoids Using HPLC-ED and Photometric Assays

Abstract

The present investigation reports on the application of a new antioxidant activity assay for the examination of flavonoids. It has been shown that the high-performance liquid chromatography with electrochemical detection (HPLC-ED) measurements allow to obtain additional information about the antioxidative properties of pure compounds by measuring their half-wave potential, the chromatographic peak height, and the product of the peak height and exponent of potential. In comparison to the classical electrochemical measurements, the HPLC-ED is characterized by a much smaller detection limit. The results were compared with the standard photometric measurement based on 1,1-diphenyl-2-picrylhydrazyl radicals. The possible antioxidant activity forecasting is also discussed. © 2013 The Author(s).