Needle morphological evidence of the homoploid hybrid origin of Pinus densata based on analysis of artificial hybrids and the putative parents, Pinus tabuliformis and Pinus yunnanensis

Abstract

Genetic analyses indicate that Pinus densata is a natural homoploid hybrid originating from Pinus tabuliformis and Pinus yunnanensis. Needle morphological and anatomical features show relative species stability and can be used to identify coniferous species. Comparative analyses of these needle characteristics and phenotypic differences between the artificial hybrids, P. densata, and parental species can be used to determine the genetic and phenotypic evolutionary consequences of natural hybridization. Twelve artificial hybrid families, the two parental species, and P. densata were seeded in a high-altitude habitat in Linzhi, Tibet. The needles of artificial hybrids and the three pine species were collected, and 24 needle morphological and anatomical traits were analyzed. Based on these results, variations in 10 needle traits among artificial hybrid families and 22 traits among species and artificial hybrids were predicted and found to be under moderate genetic control. Nineteen needle traits in artificial hybrids were similar to those in P. densata and between the two parental species, P. tabuliformis and P. yunnanensis. The ratio of plants with three needle clusters in artificial hybrids was 22.92%, which was very similar to P. densata. The eight needle traits (needle length, the mean number of stomata in sections 2 mm in length of the convex and flat sides of the needle, mean stomatal density, mesophyll/vascular bundle area ratio, mesophyll/resin canal area ratio, mesophyll/(resin canals and vascular bundles) area ratio, vascular bundle/resin canal area ratio) relative to physiological adaptability were similar to the artificial hybrids and P. densata. The similar needle features between the artificial hybrids and P. densata could be used to verify the homoploid hybrid origin of P. densata and helps to better understand of the hybridization roles in adaptation and speciation in plants. Comparative analysis of needle characteristics and phenotypic differences were conducted on artificial hybrid families, Pinus densata, and parental species (P. tabuliformis and P. yunnanensis) and the seedling grew in a high-altitude habitat in Linzhi, Tibet. Nineteen needle traits in artificial hybrid were similar to those in P. densata and between the two parent species in general, and the eight needle traits (NL, CSR2N, FSR2N, MSD, MA/VBA, MA/RCA, MA/(VBA + RCA), VBA/RCA), relative to physiological adaptability, were very similar and had the same varying tendency in artificial hybrid and P. densata. The similar needle features of artificial hybrid and P. densata were helpful to verify the homoploid hybrid origin of P. densata, with enriching the knowledge of the hybridization roles in adaptation and speciation in plants. © 2014 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.