Independent Contributions of Discrete Dopaminergic Circuits to Cellular Plasticity, Memory Strength, and Valence in Drosophila

Abstract

Dopaminergic neurons play a key role in encoding associative memories, but little is known about how these circuits modulate memory strength. Here we report that different sets of dopaminergic neurons projecting to the Drosophila mushroom body (MB) differentially regulate valence and memory strength. PPL2 neurons increase odor-evoked calcium responses to a paired odor in the MB and enhance behavioral memory strength when activated during olfactory classical conditioning. When paired with odor alone, they increase MB responses to the paired odor but do not drive behavioral approach or avoidance, suggesting that they increase the salience of the odor without encoding strong valence. This contrasts with the role of dopaminergic PPL1 neurons, which drive behavioral reinforcement but do not alter odor-evoked calcium responses in the MB when stimulated. These data suggest that different sets of dopaminergic neurons modulate olfactory valence and memory strength via independent actions on a memory-encoding brain region. Boto et al. investigated the roles of two sets of dopaminergic neurons that converge on a memory-encoding brain region in flies. While one set, PPL1, drives aversive reinforcement (valence), PPL2 neurons enhance memory strength via modulation of Ca2+ response plasticity in memory-encoding mushroom body neurons.